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- STAT5 alpha Antibodies
Invitrogen
View all (104) STAT5 alpha antibodies
Cite STAT5 alpha Polyclonal Antibody
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STAT5 alpha Antibody (PA5-81045) in IP
STAT5 alpha Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg A549 Whole Cell Lysate, Lane C: 0.5 mg Jurkat Whole Cell Lysate 1 µL with STAT5 alpha Polyclonal Antibody (Product # PA5-81045) and 15 µL of 50 % Protein G agarose. Primary antibody: STAT5 alpha Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 90 kDa. Observed band size: 9... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
PA5-81045
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:1,000
-
Immunohistochemistry (Paraffin) (IHC (P))
1:2,500-1:10,000
-
Immunocytochemistry(ICC/IF)
1:1,500-1:50,000
-
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human STAT5a
Conjugate
Unconjugated
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2788297
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human STAT5a.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form hom*o- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
UniProt ID:(Human) P42229
Entrez Gene ID:(Human) 6776
Molecular Function: 
DNA-binding transcription factor
Function(s)
allantoin metabolic processpositive regulation of endothelial cell proliferationcitrate metabolic process2-oxoglutarate metabolic processsuccinate metabolic processoxaloacetate metabolic processtranscription, DNA-templatedregulation of transcription from RNA polymerase II promoterisoleucine metabolic processvaline metabolic processcreatine metabolic processfatty acid metabolic processJAK-STAT cascadelactationpeptidyl-tyrosine phosphorylationtaurine metabolic processprolactin signaling pathwayregulation of multicellular organism growthpositive regulation of blood vessel endothelial cell migrationcreatinine metabolic processJAK-STAT cascade involved in growth hormone signaling pathway
Process(es)
DNA bindingtranscription factor activity, sequence-specific DNA bindingprotein tyrosine kinase activitysignal transducer activityprotein binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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